Clinical research
Comparison of the GenoType® MTBC Molecular Genetic Assay with culture methods in the diagnosis of tuberculosis
Comparison of the GenoType® MTBC Molecular Genetic Assay with culture methods in the diagnosis of tuberculosis
Submission date: 2011-05-02
Final revision date: 2011-12-15
Acceptance date: 2012-01-25
Online publication date: 2012-06-28
Publication date: 2014-04-30
Arch Med Sci 2014;10(2):315–318
KEYWORDS
TOPICS
ABSTRACT
Introduction: Clinical samples from 433 patients pre-diagnosed with tuberculosis in Konya, Turkey, were investigated prospectively to compare the GenoType® MTBC test (GenoType® MTBC) with conventional “gold standard” culture methods.
Material and methods: Lowenstein Jensen (LJ) and Mycobacteria Growth Indicator Tube (MGIT)-960 culture methods and GenoType® MTBC were performed together.
Results: Mycobacterium tuberculosis (M. tuberculosis) detection rates were 16.2% by culture methods, 15.4% by GenoType® MTBC, and 6% by acid-fast bacilli microscopy. The LJ or MGIT-960 with GenoType® MTBC detected M. tuberculosis in 12 samples each that were negative according to the other culture method alone. Among 70 M. tuberculosis-positive samples, detection rates were 37% (26/70) by microscopy and 82.8% (58/70) by LJ and MGIT-960, but 95.7% (67/70) by GenoType® MTBC.
Conclusions: GenoType® MTBC may be used as a beneficial adjunct test to culture methods for the detection of M. tuberculosis.
Material and methods: Lowenstein Jensen (LJ) and Mycobacteria Growth Indicator Tube (MGIT)-960 culture methods and GenoType® MTBC were performed together.
Results: Mycobacterium tuberculosis (M. tuberculosis) detection rates were 16.2% by culture methods, 15.4% by GenoType® MTBC, and 6% by acid-fast bacilli microscopy. The LJ or MGIT-960 with GenoType® MTBC detected M. tuberculosis in 12 samples each that were negative according to the other culture method alone. Among 70 M. tuberculosis-positive samples, detection rates were 37% (26/70) by microscopy and 82.8% (58/70) by LJ and MGIT-960, but 95.7% (67/70) by GenoType® MTBC.
Conclusions: GenoType® MTBC may be used as a beneficial adjunct test to culture methods for the detection of M. tuberculosis.
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