NEUROLOGY / RESEARCH PAPER
The correlation between the granulocyte colony-stimulating factor and the Notch signaling pathway in ischemic brain injury
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The First Affiliated Hospital of Nanchang University
CORRESPONDING AUTHOR
Wenyuan Xu   

The First Affiliated Hospital of Nanchang University
Submission date: 2019-11-11
Final revision date: 2020-02-14
Acceptance date: 2020-02-18
Online publication date: 2021-03-24
 
 
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ABSTRACT
Introduction:
This study aims to determine the relationship between the granulocyte colony-stimulating factor (G-CSF) and the Notch signaling pathway in ischemic brain injury.

Material and methods:
PC-12 cells were treated with the nerve growth factor (NGF) to induce neuronal differentiation then divided into seven groups: 1) no treatment (control); 2) oxygen-glucose deprivation (OGD) model; 3) overexpressed G-CSF + OGD model; 4) transfected empty vector (negative control; NC) + OGD model; 5) overexpressed G-CSF + γ-secretase inhibitor MW167 + OGD model; 6) MW167 + OGD model; and 7) NC + MW167 + OGD model. The cells were analyzed using immunohistochemistry and apoptosis and CCK8 assays. The expression of the related molecules in the Notch pathway was detected using the Western blotting and quantitative PCR (Q-PCR).

Results:
Most PC-12 cells were neuron-specific enolase (NSE)-positive after the NGF treatment. When compared with the control group, the MW167 + OGD and NC + MW167 + OGD groups had the lowest optical density (OD) values, followed by the OGD, NC + OGD and the G-CSF + MW167+ OGD groups. The G-CSF + OGD group had the highest OD value. Concerning apoptosis detection, the control group had the lowest apoptosis rate. The highest apoptosis rates were found in the MW167 + OGD, the OGD, and then the G-CSF + OGD groups.

Conclusions:
The blocking of the Notch pathway can attenuate the G-CSF effects, whereas the G-CSF overexpression can activate the Notch pathway to resist the effects of oxygen-glucose deprivation.

eISSN:1896-9151
ISSN:1734-1922