RESEARCH PAPER
Novel green synthesis of silver nanoparticles mediated
by Curcumae kwangsiensis for anti-lung cancer
activities: a preclinical trial study
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1 |
Department of Critical Care Medicine, Xi’an International Medical Center Hospital,
Xi’an, Shaanxi, China |
2 |
Department of Respiratory and Critical Care Medicine, Xian XD Group Hospital,
Lianhu District, Xi’an City, Shaanxi Province |
3 |
Department of Surgery, Dayi County People’s Hospital, Dali County, Weinan, Shaanxi,
China |
4 |
Department of Botany and Microbiology, College of Science, King Saud University,
Riyadh, Saudi Arabia |
CORRESPONDING AUTHOR
Zhiguo Zhang
Xidian Group Hospital, Xidian Group Hospital, No.97 Fengdeng Road, Lianhu, 710077, Shaanxi, China
Submission date: 2021-01-01
Final revision date: 2021-02-09
Acceptance date: 2021-03-07
Online publication date: 2021-03-18
KEYWORDS
TOPICS
ABSTRACT
Introduction:
The present work describes the green synthesis and characterization and cytotoxicity, antioxidant, and anti-human lung cancer activities of silver nanoparticles containing Curcumae kwangsiensis folium leaf
aqueous extract.
Material and methods:
Ag nanoparticles were produced by mixing the
AgNO3
solution with aqueous C. kwangsiensis folium leaf extract. Characterization of Ag nanoparticles was done by FE-SEM, FT-IR, TEM, and UV-Vis.
FE-SEM and TEM images revealed an average diameter of 15-21 nm for the
nanoparticles. MTT assay was used on common human lung cancer cell
lines, i.e., lung well-differentiated bronchogenic adenocarcinoma (HLC-1),
lung moderately differentiated adenocarcinoma (LC-2/ad), and lung poorly
differentiated adenocarcinoma (PC-14) cell lines, to survey the cytotoxicity
and anti-human lung cancer effects of Ag nanoparticles.
Results:
: They had very low cell viability and high anti-human lung cancer activities dose-dependently against HLC-1, LC-2/ad, and PC-14 cell lines without any cytotoxicity towards the normal cell line (HUVEC). The IC50 values of
Ag nanoparticles were 249, 187, and 152 µg/ml against HLC-1, LC-2/ad, and
PC-14 cell lines, respectively. The best results of cytotoxicity and anti-human lung cancer properties were seen at the concentration of 1000 µg/ml.
Ag nanoparticles inhibited half of the DPPH molecules in the concentration
of 135 µg/ml. Maybe significant anti-human lung cancer potentials of Ag
nanoparticles synthesized by C. kwangsiensis folium leaf aqueous extract
against common human lung cancer cell lines are linked to their antioxidant
activities.
Conclusions:
After confirming the above results in the clinical trial research,
this formulation can be administered to treat human lung cancers in humans.