OBSTETRICS AND GYNAECOLOGY / RESEARCH PAPER
 
KEYWORDS
TOPICS
ABSTRACT
Introduction:
To explore the mechanism and clinical effect of acupoint selection and acupuncture therapy on premature ovarian failure.

Material and methods:
A total of 100 cases of POF were collected and divided into POF group and POF+acupoint selection and acupuncture therapy (POF+ACU) group. Cerebrospinal fluid samples from patients in each group were extracted by lumbar puncture, and exosomes were isolated and purified. The expression level of exosome protein NELL2 was analyzed by western blot, and the morphology of exosomes was observed by transmission electron microscopy (TEM). The changes of sex hormone levels were detected by TOSOH AIA 1800 automatic chemiluminescence analyzer. Follicular development and ovulation were monitored by B-ultrasound.

Results:
The expression of protein CD9, CD81 and NELL2 in POF+ACU group all increased compared with POF group. TEM images showed that the exosomes were oval or round membranous vesicles. After 3 months of continuous acupoint selection and acupuncture therapy, it was found that the level of testosterone (T) and estradiol (E2) in the POF+ACU group were significantly increased, while the follicular stimulating hormone (FSH) and luteinizing hormone (LH) were significantly decreased, showing a significant difference compared with the POF group. B-ultrasound observation of the endometrial thickness and the maximum follicle diameter showed that the values of POF+ACU group increased, showing no statistical difference compared with the NC group. Ovulation occurred in 20% of the 50 patients in the POF group and 70% in the POF+ACU treatment group.

Conclusions:
Acupoint selection and acupuncture therapy can treat POF by promoting the secretion of exosome protein NELL2

eISSN:1896-9151
ISSN:1734-1922