PHARMACOLOGY AND PHARMACY / EXPERIMENTAL RESEARCH
 
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Diosmin is a natural citrus flavone with remarkable antioxidant and anti-inflammatory features. Acute leukemia is a common type of cancer that is caused in the blood.

Material and methods:
α-Amylase activity was determined by a method adapted from the work of Taha et al.

Results:
In this study, we examined its effect on some important enzymes, the IC50 values were 196.07 for Aldose reductase, and 76.40 for α-Amylase. The molecular docking study was performed to assess the binding affinity and biological activities of diosmin in the presence of alpha amylase and aldose reductase.

Discussion:
The results of the docking study indicated that diosmin has a remarkable binding affinity to these enzymes with a docking score of –9.768 and –140469 for α-amylase and aldose reductase, respectively. Therefore, this compound could be used as a potential inhibitor for these enzymes. In the cellular and molecular part of the recent study, the cells treated with diosmin were assessed by MTT assay for 48 h regarding the cytotoxicity and anti-human acute lymphoblastic leukemia properties on HL-60, Clone 15 HL-60, HL-60/MX1, and HL-60/MX2 cell lines. The IC50 values of diosmin were 466, 323, 502, and 537 µg/ml against HL-60, Clone 15 HL-60, HL-60/MX1, and HL-60/MX2 cell lines, respectively.

Conclussions:
The viability of acute lymphoblastic leukemia cell lines decreased dose-dependently in the presence of diosmin. It appears that the anti-human acute lymphoblastic leukemia effect of diosmin is due to its antioxidant effects.

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ISSN:1734-1922