EXPERIMENTAL RESEARCH
Involvement of PI3K/Akt/β-catenin signaling in schisandrin B-mitigated bone deterioration in an experimental rat model of estrogen deficiency
,
 
Wei Li 2
,
 
,
 
 
 
 
More details
Hide details
1
Department of Orthopedics, The First People’s Hospital of Taizhou, Taizhou, China
2
The Second Clinical School of North Sichuan Medical College, Nanchong, China
3
Department of Spine Surgery, Affiliated Hospital of Yanbian University, Yanji, China
Submission date: 2019-07-10
Final revision date: 2019-09-20
Acceptance date: 2019-10-02
Online publication date: 2020-02-11
 
 
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Schisandrin B (SchB) has been reported to perform a wide range of biological functions, including antioxidant activity, anti-inflammatory activity and stimulation of osteoblast proliferation. However, the function and mechanism of SchB in ovariectomy (OVX)-induced osteoporosis are still unknown. The present study was designed to investigate the anti-osteoporotic activity of SchB in an experimental rat model of estrogen deficiency, which is usually used to mimic human postmenopausal osteoporosis (PMO).

Material and methods:
OVX rats were orally treated with low (10 mg/kg) or high (50 mg/kg) doses of SchB for 8 weeks. Bone metabolism-related markers were measured by ELISA. The levels of protein expression were determined by western blotting analysis. Hematoxylin and eosin (H&E) and safranin O staining were performed to analyze trabecular bone and cartilage degeneration. Tartrate-resistant acid phosphatase (TRAP) staining was used to evaluate osteoclast differentiation.

Results:
SchB administration markedly increased serum Ca levels and bone Ca content and decreased urinary calcium excretion in OVX-operated rats. In addition, high-dosage SchB treatment blocked osteoclastogenesis and improved trabecular bone and cartilage degeneration in the tibia of OVX-operated rats. Furthermore, high-dosage SchB treatment dramatically elevated the protein expression of phospho-PI3K, phospho-Akt and β-catenin in OVX-operated rats.

Conclusions:
SchB exerted anti-osteoporotic activity in OVX-operated rats by accelerating the phosphorylation of PI3K and Akt, subsequently upregulating the expression of β-catenin.

eISSN:1896-9151
ISSN:1734-1922