OBSTETRICS AND GYNAECOLOGY / RESEARCH PAPER
 
KEYWORDS
TOPICS
ABSTRACT
Introduction:
To identify circular RNAs (circRNAs) expression profiles in the blood of preeclampsia (PE) and healthy pregnant women, further clarify the possible mechanisms of circRNAs involved in the pathogenesis of PE.

Material and methods:
Methods: Whole blood samples were collected from 5 paired PE and healthy pregnant women, the differentially expressed circRNAs (DE-circRNAs) were investigated by using high-throughput sequencing. Bioinformatics was performed to evaluate the sequencing results and obtain insights into possible mechanisms, such as GO and KEGG pathway analyses. Then, six DE-circRNAs were chosen and validated by quantitative real-time PCR (RT-qPCR) in an enlarged sample size. Their diagnostic values were analyzed by the receiver operating characteristic (ROC) curve. The establishment and analysis of the circRNA-miRNA-mRNA network were made for the validated DE-circRNAs.

Results:
Results: A total of 139 DE-circRNAs between PE and controls were revealed. Of them, 18 circRNAs were upregulated, and 31 circRNAs were downregulated (fold change >2 and P-value < 0.05). Three circRNAs (has-circ-0007717, has-circ-0006460, and has-circ-0093055) were higher in both blood of early-onset and late-onset PE patients. The ROC analysis showed area under the curve values of has-circ-0007717, has-circ-0006460, and has-circ-0093055 were 0.64 (P=0.11), 0.72 (P=0.01), and 0.72 (P=0.01), respectively. Then, the circRNA-miRNA-mRNA competing for endogenous RNAs (ceRNAs) network comprised 2 circRNAs, 154 miRNAs, and 6 mRNAs. KEGG analysis of these mRNAs included immunity response-related pathways and cellular senescence.

Conclusions:
Conclusion: CeRNA regulatory network indicated that the DE-circRNAs might participate in the processes of cell immunity response.

eISSN:1896-9151
ISSN:1734-1922