EXPERIMENTAL RESEARCH
A study on inhibition of the Aβ1-42-induced inflammatory response by the Huatuo Zaizao pill through the NF-κB signaling pathway
Yun Xu
1,4,6
| 1 | Department of Neurology, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210008, P. R. China |
| 2 | Department of Neurology, Jiangsu Taizhou People’s Hospital, Taizhou, Jiangsu 225300, P. R. China |
| 3 | Department of Neurology, Jiangsu Taizhou People’s Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 225300, P. R. China |
| 4 | Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210008, P. R. China |
| 5 | Department of Neurology, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210001, P. R. China |
| 6 | Jiangsu Key Laboratory for Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, P. R. China |
Submission date: 2019-07-05
Final revision date: 2019-09-23
Acceptance date: 2019-10-15
Online publication date: 2020-10-21
Arch Med Sci 2023;19(4)
KEYWORDS
Alzheimer’s diseaseamyloid-βmicrogliainflammationNF-κB signaling pathwayamyloid-NF-B signaling pathway
TOPICS
ABSTRACT
Introduction:
The pathology of Alzheimer’s disease (AD) includes -amyloid (A) (plaques) and neurofibrillary tangles (NFTs). This study aimed to explore the efficacy of Huatuo Zaizao pill (HTZP) in an AD mouse model induced by injecting A1-42, and the neuroprotective mechanism of HTZP in AD.
Material and methods:
C57BL/6 (B6) mice were randomly divided into 4 groups (n = 10, per group): control group, AD model group, and 2 different doses of HTZP treated groups. The Morris water maze test was carried out on AD mice to assess the learning ability after treatment with HTZP for 15 day. The levels of inflammatory factors and the nuclear factor-B (NF-B) pathway were examined by western blot and real-time polymerase chain reaction (PCR). The content of microglia was investigated by immunofluorescence.
Results:
This study revealed that a cognitive disorder could be mitigated when the AD mice were treated with HTZP, which might be associated with the decreased level of pro-inflammatory factors, and the inhibitory activities of microglia. Additionally, phosphorylation of IB and NF-B p65 could be reduced by prohibiting the neuroinflammation of NF-B activation in the hippocampus of AD mice.
Conclusions:
These results showed that HTZP could mitigate a cognitive disorder, diminish the activation of microglia, and inhibit the content of inflammatory factors through the NF-B pathway in A1-42-induced AD mice. HTZP may be an appropriate agent for AD treatment in the future.
The pathology of Alzheimer’s disease (AD) includes -amyloid (A) (plaques) and neurofibrillary tangles (NFTs). This study aimed to explore the efficacy of Huatuo Zaizao pill (HTZP) in an AD mouse model induced by injecting A1-42, and the neuroprotective mechanism of HTZP in AD.
Material and methods:
C57BL/6 (B6) mice were randomly divided into 4 groups (n = 10, per group): control group, AD model group, and 2 different doses of HTZP treated groups. The Morris water maze test was carried out on AD mice to assess the learning ability after treatment with HTZP for 15 day. The levels of inflammatory factors and the nuclear factor-B (NF-B) pathway were examined by western blot and real-time polymerase chain reaction (PCR). The content of microglia was investigated by immunofluorescence.
Results:
This study revealed that a cognitive disorder could be mitigated when the AD mice were treated with HTZP, which might be associated with the decreased level of pro-inflammatory factors, and the inhibitory activities of microglia. Additionally, phosphorylation of IB and NF-B p65 could be reduced by prohibiting the neuroinflammation of NF-B activation in the hippocampus of AD mice.
Conclusions:
These results showed that HTZP could mitigate a cognitive disorder, diminish the activation of microglia, and inhibit the content of inflammatory factors through the NF-B pathway in A1-42-induced AD mice. HTZP may be an appropriate agent for AD treatment in the future.
RELATED ARTICLE
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.