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PHARMACOLOGY AND PHARMACY / BASIC RESEARCH
Anti-ovarian cancer and collagenase, α-amylase, and aldose reductase inhibition properties of 2'-hydroxy-5'-methoxyacetophenone with molecular modeling studies
1
Department of Gynecology, Shandong Second Provincial General Hospital, Jinan City, Shandong Province, China
2
Department of Gynecology, Shandong Provincial Maternal and Child Health Care Hospital, Shandong University, Jinan City, Shandong Province, China
Submission date: 2021-04-16
Final revision date: 2021-09-07
Acceptance date: 2021-09-19
Online publication date: 2021-09-28
Corresponding author
Yuanyuan Zhi
Department of Gynecology, Shandong Provincial Maternal and Child Health Care Hospital, Shandong University, No. 238 Jingshi East Road, Jinan City, Shandong Province, 250000, China, China
Department of Gynecology, Shandong Provincial Maternal and Child Health Care Hospital, Shandong University, No. 238 Jingshi East Road, Jinan City, Shandong Province, 250000, China, China
KEYWORDS
TOPICS
ABSTRACT
Introduction:
This work investigated the inhibition effect of 2'-hydroxy-5'-methoxyacetophenone on aldose reductase, α-amylase, and also collagenase enzymes. Also, molecular modeling calculations were performed comparing the biochemical activity of the 2'-hydroxy-5'-methoxyacetophenone molecule with these enzymes.
Material and methods:
The enzymes used in these calculations are aldose reductase (AR), α-amylase (Alpha-Amy), and collagenase from Clostridium histolyticum (Coll). After the docking calculations were performed, ADME/T analysis parameters were examined in order to calculate the properties of the methoxyacetophenone molecule to be utilized as a drug in the future. For determining anti-ovarian cancer properties of 2'-hydroxy-5'-methoxyacetophenone, the MTT assay was used on normal (HUVEC), PA-1, Caov-3, and SK-OV-3 cell lines.
Results:
The IC50 values of 2'-hydroxy-5'-methoxyacetophenone against PA-1, Caov-3, and SK-OV-3 cell lines were found to be 271, 326 and 405 µg/ml, respectively.
Conclusions:
2'-hydroxy-5'-methoxyacetophenone was found to have significant antioxidant and anti-ovarian cancer properties. It appears that the anti-ovarian cancer effect of 2'-hydroxy-5'-methoxyacetophenone is due to its antioxidant effects.
This work investigated the inhibition effect of 2'-hydroxy-5'-methoxyacetophenone on aldose reductase, α-amylase, and also collagenase enzymes. Also, molecular modeling calculations were performed comparing the biochemical activity of the 2'-hydroxy-5'-methoxyacetophenone molecule with these enzymes.
Material and methods:
The enzymes used in these calculations are aldose reductase (AR), α-amylase (Alpha-Amy), and collagenase from Clostridium histolyticum (Coll). After the docking calculations were performed, ADME/T analysis parameters were examined in order to calculate the properties of the methoxyacetophenone molecule to be utilized as a drug in the future. For determining anti-ovarian cancer properties of 2'-hydroxy-5'-methoxyacetophenone, the MTT assay was used on normal (HUVEC), PA-1, Caov-3, and SK-OV-3 cell lines.
Results:
The IC50 values of 2'-hydroxy-5'-methoxyacetophenone against PA-1, Caov-3, and SK-OV-3 cell lines were found to be 271, 326 and 405 µg/ml, respectively.
Conclusions:
2'-hydroxy-5'-methoxyacetophenone was found to have significant antioxidant and anti-ovarian cancer properties. It appears that the anti-ovarian cancer effect of 2'-hydroxy-5'-methoxyacetophenone is due to its antioxidant effects.
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| eISSN: | 1896-9151 |
| ISSN: | 1734-1922 |
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