Basic research
Effect of low-intensity pulsed ultrasound on l929 fibroblasts
Effect of low-intensity pulsed ultrasound on l929 fibroblasts
,
,
Submission date: 2009-10-09
Final revision date: 2009-12-18
Acceptance date: 2010-01-18
Online publication date: 2011-05-17
Publication date: 2011-04-29
Arch Med Sci 2011;7(2):224–229
KEYWORDS
TOPICS
ABSTRACT
Introduction : Ultrasound has proven to be an important therapeutic resource regarding musculoskeletal disease and is routinely used in physical therapy and medicine both therapeutically and diagnostically. The aim of the present study was to analyse the effects with different ultrasound intensities in order to establish the ideal radiation level in cell cultures.
Material and methods : Fibroblast cell cultures were divided into five groups: group I – control (did not receive irradiation); group II – 0.2 W/cm2 in pulsed mode at 10% (1 : 9 duty cycle); group III – 0.6 W/cm2 in pulsed mode at 10% (1 : 9 duty cycle); group IV – 0.2 W/cm2 in pulsed mode at 20% (2 : 8 duty cycle); and group V – 0.6 W/cm2 in pulsed mode at 20% (2 : 8 duty cycle). Each group was irradiated with 24-h intervals, observing the following post-irradiation incubation times: 24, 48, 72 and 96 h; after 24 h of each irradiation, cultures were analysed using the MTT method.
Results : Analysis of the results following ultrasound irradiation demonstrated that the effect of ultrasound with 0.6 W/cm2 in pulsed mode at 10% (1 : 9 duty cycle) was statistically significant in relation to ultrasonic irradiation in pulsed mode at 20% (2 : 8 duty cycle) (p < 0.05).
Conclusions : According to parameters used in the irradiation of cultivated fibroblasts, the pulse mode regime and the control of intensity are of fundamental importance for the optimal use of therapeutic ultrasound. Furthermore, low and medium intensities decreased cell damage, which establishes that acoustic pulsed energy induces the proliferation of fibroblast cells.
Material and methods : Fibroblast cell cultures were divided into five groups: group I – control (did not receive irradiation); group II – 0.2 W/cm2 in pulsed mode at 10% (1 : 9 duty cycle); group III – 0.6 W/cm2 in pulsed mode at 10% (1 : 9 duty cycle); group IV – 0.2 W/cm2 in pulsed mode at 20% (2 : 8 duty cycle); and group V – 0.6 W/cm2 in pulsed mode at 20% (2 : 8 duty cycle). Each group was irradiated with 24-h intervals, observing the following post-irradiation incubation times: 24, 48, 72 and 96 h; after 24 h of each irradiation, cultures were analysed using the MTT method.
Results : Analysis of the results following ultrasound irradiation demonstrated that the effect of ultrasound with 0.6 W/cm2 in pulsed mode at 10% (1 : 9 duty cycle) was statistically significant in relation to ultrasonic irradiation in pulsed mode at 20% (2 : 8 duty cycle) (p < 0.05).
Conclusions : According to parameters used in the irradiation of cultivated fibroblasts, the pulse mode regime and the control of intensity are of fundamental importance for the optimal use of therapeutic ultrasound. Furthermore, low and medium intensities decreased cell damage, which establishes that acoustic pulsed energy induces the proliferation of fibroblast cells.
RELATED ARTICLE
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.