ONCOLOGY / RESEARCH PAPER
Correlation between KRAS, NRAS and BRAF mutations
and tumor localizations in patients with primary
and metastatic colorectal cancer
More details
Hide details
1 |
Department of Pneumonology, Oncology and Allergology, Medical University
of Lublin, Lublin, Poland |
2 |
Institute of Genetics and Immunology GENIM LLC, Lublin, Poland |
3 |
Department of Oncology and Chemotherapy, Medical University of Lublin, Lublin,
Poland |
4 |
Department of Oncology, Medical University of Poznań, Poznań, Poland |
CORRESPONDING AUTHOR
Aleksandra Bożyk
Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, 20-950 Lublin, Poland
Submission date: 2018-12-12
Final revision date: 2019-04-23
Acceptance date: 2019-05-06
Online publication date: 2021-03-24
Arch Med Sci 2022;18(6)
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Detection of abnormalities in the KRAS, NRAS and BRAF genes
is extremely important for proper qualification of colorectal cancer (CRC) patients for therapy with anti-EGFR (epidermal growth factor receptor) monoclonal antibodies. However, data about prevalence of mutations in these
genes, in different localizations of CRC tumors, are limited.
Material and methods:
We examined the frequency of mutations in the
KRAS, NRAS and BRAF genes in 500 Caucasian CRC patients (200 women
and 300 men, median age 66 years). DNA was isolated from formalin-fixed,
paraffin-embedded (FFPE) tissues using a Qiagen QIAamp DNA FFPE-kit.
Analysis of mutations was carried out using the KRAS/BRAF, NRAS and BRAF
Mutation Analysis Kit for Real-Time PCR (EntroGen) with the Cobas 480 realtime PCR apparatus (Roche Diagnostics).
Results:
KRAS mutations were detected in 190 patients (38%), NRAS mutations in 20 patients (4%), and BRAF mutations in 24 patients (4.8%). There
were no associations between age of CRC patients and frequency of KRAS,
NRAS and BRAF gene mutations. These mutations were significantly more
often diagnosed in women (55.5%) than in men (41%, p < 0.005). Tumors of
the rectum and sigmoideum were the most often observed in both groups
of CRC patients – with and without KRAS, NRAS and BRAF gene mutations.
However, transverse colon, ascending colon and cecum cancers were the
most often affected by mutations.
Conclusions:
Our study showed that the occurrence of mutations in the
KRAS, NRAS and BRAF genes is not accidental and depends on the location
of CRC tumors.