Endothelium-dependent relaxation induced by etomidate in the aortas of insulin-resistant rats
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Submission date: 2016-08-25
Final revision date: 2016-12-01
Acceptance date: 2016-12-15
Online publication date: 2018-08-13
Publication date: 2018-08-07
Arch Med Sci 2018;14(5):1155-1162
Few reports have mentioned the effect of etomidate on the aortas of insulin-resistant (IR) rats. In this study, we investigated the effect of etomidate on isolated IR aortas of rats, and explored its underlying mechanism.

Material and methods:
The IR rat model was established through feeding with a high-fructose diet. The systolic blood pressure (SBP) was measured by the tailcuff method before grouping and at the end of the 8-week feeding; blood samples were also obtained for analysis. Thoracic aorta rings of IR rats were isolated and suspended in a tissue bath. The tensile force was recorded isometrically. The effect of etomidate on provoked contraction of the rings was assessed with or without a potassium channel blocker or NO synthase inhibitor

Etomidate-induced relaxation in IR rings was greater than normal control (NC) rings (all p < 0.001 with etomidate log M of –4 to –6). NG-nitro- L-arginine methyl ester (L-NAME, an NO synthase inhibitors) inhibited etomidate-induced relaxation in NC rings, but had no effect on the IR rings (all p < 0.001 with etomidate log M of –4 to –6). Pre-incubation with glibenclamide (Gli, a potassium channel blocker) significantly inhibited etomidate- induced relaxation in NC and IR rings (all p < 0.001 with etomidate log M of –4 to –6), and had no inhibited effect on endothelial denuded aortic rings.

IR increased etomidate-induced relaxation in rat aortas. Etomidate causes vasodilation in IR rat aortas via both endothelium-dependent and independent ways; impaired NO-mediated relaxation was disrupted and ATP-sensitive potassium (KATP) channel-mediated relaxation may be involved in the endothelium-dependent relaxation of etomidate in IR rats.

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