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ANESTHESIOLOGY / CLINICAL RESEARCH
Levels of small extracellular vesicles in patients treated with hyperbaric oxygenation
1
Department of Hyperbaric Medicine, Military Institute of Medicine – National Research Institute, Poland
2
Department of Biochemistry, Medical University of Warsaw, Poland
3
National Centre for Hyperbaric Medicine, Institute of Maritime and Tropical Medicine, Medical University of Gdansk, Poland
4
Department of Oral and Maxillofacial Surgery, University Hospital Regensburg, Germany
5
Department of Internal Medicine and Endocrinology, Medical University of Warsaw, Poland
Submission date: 2023-05-17
Final revision date: 2023-07-06
Acceptance date: 2023-07-08
Online publication date: 2023-07-25
Corresponding author
Natalia Jermakow
Department of Hyperbaric Medicine, Military Institute of Medicine – National Research Institute, Szaserów 128, 04-141, Warsaw, Poland
Department of Hyperbaric Medicine, Military Institute of Medicine – National Research Institute, Szaserów 128, 04-141, Warsaw, Poland
Arch Med Sci 2024;20(2):476-484
KEYWORDS
exosomeshyperbaric oxygen therapysmall extracellular vesiclesnecrotizing soft-tissue infectionsaseptic bone necrosisidiopathic sudden sensory neural hearing loss
TOPICS
ABSTRACT
Introduction:
Hyperbaric oxygen (HBO2) therapy involves the inhalation of pure oxygen in a pressure chamber under increased ambient pressure. Recent research indicates that circulating small extracellular vesicles (sEVs) play important roles in human physiology and pathology. Therefore, the objective of this pilot study was to monitor the impact of HBO2 therapy on the levels of circulating sEVs in the serum of patients with necrotizing soft-tissue infections (NSTI), aseptic bone necrosis (ABN) or idiopathic sudden sensory neural hearing loss (ISSNHL).
Material and methods:
Serum-derived sEVs were isolated and quantified in 80 patients before and after HBO2 therapy applied for NSTI, ISSNHL and ABN patients as well as in normal controls who received neither HBO2 therapy nor steroids.
Results:
We observed a significant increase of circulating sEVs in patients with ISSNHL after HBO2 therapy (p < 0.05), as well as significantly elevated levels of sEVs after HBO2 therapy compared to patients with NSTI (p < 0.05) and ABN (p < 0.01).
Conclusions:
The increase in the levels of sEVs in ISSNHL may be evidence for both the intended reduction of inflammation as a result of steroid therapy and the inhibitory effect of oxidative stress induced by HBO2 therapy. Thus, sEVs released during HBO2 therapy might play an important biological role in mediating the response to therapy and might be a promising approach to gain further insights into the therapeutic efficacy of HBO2 therapy.
Hyperbaric oxygen (HBO2) therapy involves the inhalation of pure oxygen in a pressure chamber under increased ambient pressure. Recent research indicates that circulating small extracellular vesicles (sEVs) play important roles in human physiology and pathology. Therefore, the objective of this pilot study was to monitor the impact of HBO2 therapy on the levels of circulating sEVs in the serum of patients with necrotizing soft-tissue infections (NSTI), aseptic bone necrosis (ABN) or idiopathic sudden sensory neural hearing loss (ISSNHL).
Material and methods:
Serum-derived sEVs were isolated and quantified in 80 patients before and after HBO2 therapy applied for NSTI, ISSNHL and ABN patients as well as in normal controls who received neither HBO2 therapy nor steroids.
Results:
We observed a significant increase of circulating sEVs in patients with ISSNHL after HBO2 therapy (p < 0.05), as well as significantly elevated levels of sEVs after HBO2 therapy compared to patients with NSTI (p < 0.05) and ABN (p < 0.01).
Conclusions:
The increase in the levels of sEVs in ISSNHL may be evidence for both the intended reduction of inflammation as a result of steroid therapy and the inhibitory effect of oxidative stress induced by HBO2 therapy. Thus, sEVs released during HBO2 therapy might play an important biological role in mediating the response to therapy and might be a promising approach to gain further insights into the therapeutic efficacy of HBO2 therapy.
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| eISSN: | 1896-9151 |
| ISSN: | 1734-1922 |
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