MiR-93 suppresses cell proliferation and invasion by targeting ZNF322 in human hepatocellular carcinoma
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The Second Affiliated Hospital of Xi’an Jiaotong University
Department of Anesthesiology, Weinan Central Hospital
Jianrui Lv   

The Second Affiliated Hospital of Xi’an Jiaotong University
Submission date: 2019-01-08
Final revision date: 2019-02-18
Acceptance date: 2019-03-05
Online publication date: 2021-03-19
Arch Med Sci 2022;18(6)
This study aimed to investigate the biological role of microRNA 93 (miR-93), a novel tumor-related miRNA, in human hepatocellular carcinoma (HCC) and elucidate the potential molecular mechanisms involved.

Material and methods:
Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to determine the expression of miR-93 in HCC tissues and cell lines. The log-rank test and Kaplan-Meier survival analysis were performed to evaluate the relationship between miR-93 expression and overall survival. MTT assay, colony formation assay, Transwell migration and invasion assays were carried out to exam cell proliferation, colony formation, migration and invasion, respectively. Murine xenograft models were established to the effect of miR-93 on tumor growth in vivo. TargetScan online software was applied to predict the potential target of miR-93. Luciferase reporter assays were used to validate the direct binding of miR-93 and its putative target.

Here we found that miR-93 was significantly down-regulated in HCC tissues and cell lines. Patients with decreased miR-93 expression had a significantly shorter overall survival. Functional investigations demonstrated miR-93 over-expression suppressed HCC cell proliferation, weakened clonogenic ability, and slowed down cell migration and invasion; whereas miR-93 depletion facilitated HCC cell proliferation, colony formation, cell migration and invasion. MiR-93 over-expression retarded tumor growth in vivo. Luciferase reporter assay and rescue assay revealed that zinc finger protein 322 (ZNF322) was a direct target of miR-93 and mediated the inhibitory effects of miR-93 on HCC cell proliferation and motility.

Our data may provide some evidence for miR-93/ZNF322 axis a candidate therapeutic target for HCC.