One of the plants that has long been considered by humans is Equisetum arvense L Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders.

Material and methods:
In the current study, the antioxidant, cytotoxicity, and anti-human ling cancer properties of Equisetum arvense were investigated in the in vitro condition. Total phenolic content, total flavonoid content, radical scavenging activity, and ferrous ion chelating were run to evaluate the antioxidant activity. MTT assay was chosen to investigate anticancer activity of the plant extract.

The plant extract scavenged DPPH as a free radical with an IC50 of 12.3±0.7 µg/mL better than positive controls. The plant also was rich in phenolic compounds with an amount of 396.2±3.2 mg GAE/g for total phenolic content. In the MTT assay, human colorectal carcinoma (HCT-8 [HRT-18], Ramos.2G6.4C10, HT-29, and HCT 116) and normal cell lines (HUVEC) were used to study the cytotoxicity and anticancer potential of human colorectal over the Equisetum arvense L. The cell viability of Equisetum arvense L was very low against human colorectal carcinoma cell lines without any cytotoxicity on the normal (HUVEC) cell line.

The best anti-human colorectal carcinoma properties of Equisetum arvense L against the above cell lines was in the case of HT 29 cell line.