RESEARCH PAPER

Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma
More details
Hide details
1 |
Department of Gastrointestinal Surgery, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China |
2 |
Clinical Laboratory, the Second People’s Hospital of Nantong, Nantong, Jiangsu Province, China |
3 |
Department of Gastrointestinal surgery, Huangshi Central Hospital, affiliated hospital of Hubei polytechnic university, Edong Healthcare Group, Huangshi City, Hubei Province, China |
4 |
Biology Department, College of Science, King Khalid University, Abha, Saudi Arabia |
5 |
Zoology Department, College of Science, Damanhour University, Damanhour, Egypt |
6 |
Pathology Department, College of Medicine, Taif University, Taif, Saudi Arabia |
CORRESPONDING AUTHOR
Haiping Luo
Huangshi Central Hospital of Edong Healthcare Group, 435000, Huangshi City, China
Submission date: 2021-03-29
Final revision date: 2021-05-19
Acceptance date: 2021-05-24
Online publication date: 2021-06-08
KEYWORDS
TOPICS
ABSTRACT
Introduction:
One of the plants that has long been considered by humans is Equisetum arvense L. Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders. In the current study, the antioxidant, cytotoxicity, and anti-human lung cancer properties of Equisetum arvense were investigated in in vitro conditions.
Material and methods:
Total phenolic content, total flavonoid content, radical scavenging activity, and ferrous ion chelating were assessed to evaluate the antioxidant activity. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was chosen to investigate anticancer activity of the plant extract.
Results:
The plant extract scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a free radical with an IC50 of 12.3 ±0.7 µg/ml better than positive controls. The plant was also rich in phenolic compounds with an amount of 396.2 ±3.2 mg GAE/g for total phenolic content. In the MTT assay, human colorectal carcinoma (HCT-8 [HRT-18], Ramos.2G6.4C10, HT-29, and HCT 116) and normal cell lines (HUVEC) were used to study the cytotoxicity and anticancer potential of Equisetum arvense L against human colorectal cancer.
Conclusions:
The cell viability of Equisetum arvense L was very low against human colorectal carcinoma cell lines without any cytotoxicity towards the normal (HUVEC) cell line. The best anti-human colorectal carcinoma properties of Equisetum arvense L against the above cell lines were observed in the case of the HT 29 cell line.