Identification of key genes related to heart failure by analysis of expression profiles
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Department of Cardiology, Henan Provincial People’s Hospital, Fuwai Central China
Cardiovascular Hospital, People’s Hospital of Zhengzhou University, Zhengzhou,
Henan, China |
CORRESPONDING AUTHOR
Che Wang
Department of Cardiology, Henan Provincial People’s Hospital, Fuwai Central China Cardiovascular Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, 450003, China
Submission date: 2019-06-21
Final revision date: 2019-10-09
Acceptance date: 2019-11-28
Online publication date: 2021-03-10
KEYWORDS
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ABSTRACT
Introduction:
To elucidate the candidate biomarkers involved in the pathogenesis process of heart failure (HF) via analysis of differentially expressed
genes (DEGs) of the dataset from the Gene Expression Omnibus (GEO).
Material and methods:
The GSE76701 gene expression profiles regarding
the HF and control subjects were respectively analysed. Briefly, DEGs were
firstly identified and subjected to Cytoscape plug-in ClueGO + CluePedia and
Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses.
A protein-protein interaction (PPI) network was then built to analyse the interaction between DEGs, followed by the construction of an interaction network by combining with hub genes with the targeted miRNA genes of DEGs
to identify the key molecules of HF. In addition, potential drugs targeting key
DEGs were sought using the drug-gene interaction database (DGIdb), and
a drug-mRNA-miRNA interaction network was also constructed.
Results:
A total of 489 DEGs were verified between HF and control, which
mainly enriched in type I interferon and leukocyte migration according to
molecular function. Significantly increased levels of GAPDH, GALM1, MMP9,
CCL5, and GNAL2 were found in the HF setting and were identified as
the hub genes based on the PPI network. Furthermore, according to the
drug-mRNA-miRNA network, FCGR2B, CCND1, and NF-κb, as well as corresponding miRNA-605-5p, miRNA-147a, and miRNA-671-5p were identified
as the drug targets of HF.
Conclusions:
The hub genes GAPDH, GALM1, MMP9, CCL5, and GNAL2 were
significantly increased in HF. miRNA-605-5p, miRNA-147a, and miRNA-671-5p
were predicted as the drug target-interacted gene-miRNA of HF.