AUTOIMMUNITY / RESEARCH PAPER
Serum antinuclear autoantibodies are associated with measures of oxidative stress and lifestyle factors - analysis of LIPIDOGRAM2015 and LIPIDOGEN2015 studies.
| 1 | Euroimmun Polska Sp. z o.o., Poland |
| 2 | Department of Biochemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland |
| 3 | Department of Hypertension, Medical University of Lodz, Poland |
| 4 | Department of Family Medicine, Jagiellonian University Medical College, Poland |
| 5 | Mastej Medical Center, Poland |
| 6 | Department of Pharmacological Sciences University of Milano, Italy |
| 7 | Multimedica IRCCS, Italy |
| 8 | Imperial Centre for Cardiovascular Disease Prevention, Department of Primary Care and Public Health, Imperial College, United Kingdom |
| 9 | Department of Clinical Biochemistry, Royal Free Hospital, University College London, United Kingdom |
| 10 | Cicarrone Center for the Prevention of Cardiovascular Disease, Johns Hopkins University School of Medicine, United States |
| 11 | CGH Medical Center, United States |
| 12 | Department of Biostatistics, School of Public Health of Alabama at Birmingham, United States |
| 13 | Liverpool Centre for Cardiovascular Science, University of Liverpool, United Kingdom |
| 14 | Liverpool Heart & Chest Hospital, United Kingdom |
| 15 | Department of Clinical Medicine, Aalborg University, Denmark |
| 16 | Division of Cardiovascular Sciences, Faculty of Biology, Medicine and Health, University of Manchester, United Kingdom |
| 17 | School of Health and Life Sciences, Federation University Australia, Australia |
| 18 | Institute of Cardiovascular and Medical Science, University of Glasgow, United Kingdom |
| 19 | NIHR University College London Biomedical Research Centre, University College London, United Kingdom |
| 20 | University College London Hospitals NHS Foundation Trust, United Kingdom |
| 21 | MEMO Research, University of Dundee, United Kingdom |
| 22 | Ninewells Hospital and Medical School, United Kingdom |
| 23 | School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, United Kingdom |
| 24 | Liverpool Centre for Cardiovascular Science, United Kingdom |
| 25 | Department of Family Medicine and Public Health, Faculty of Medicine, University of Opole, Poland |
CORRESPONDING AUTHOR
Submission date: 2021-06-23
Acceptance date: 2021-06-23
Online publication date: 2021-07-03
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Oxidative stress is one of many factors suspected to promote antinuclear autoantibody (ANA) formation. Reactive oxygen species can induce changes in the antigenic structure of macromolecules, causing the immune system to treat them as “neo-antigens” and start production of autoantibodies. This study was designed to evaluate the relationship between oxidative stress markers, lifestyle factors and the detection of ANA.
Material and methods:
We examined measures of oxidative stress indices of free-radical damage to lipids and proteins, such as total oxidant status (TOS), concentration of protein thiol groups (PSH), and malondialdehyde (MDA), activity of superoxide dismutase (SOD) in in 1731 serum samples. The parameters of the non-enzymatic antioxidant system, such as total antioxidant status (TAS) and uric acid concentration (UA), were also measured and the oxidative stress index (OSI -index) was calculated. All samples were tested for the presence of ANA using an indirect immunofluorescence assay (IIFA).
Results:
The presence of ANA in women was associated with lower physical activity (p=0.036), less frequent smoking (p=0.007) and drinking of alcohol (p=0.024) accompanied by significant changes in SOD isoenzymes activity (p<0.001) and a higher uric acid (UA) concentration (p<0.001). In ANA positive males we observed lower concentrations of PSH (p=0.046) and increased concentrations of MDA (p=0.047).
Conclusions:
The results indicate that local oxidative stress may be associated with increased probability of ANA formation in a sex-specific manner.
Oxidative stress is one of many factors suspected to promote antinuclear autoantibody (ANA) formation. Reactive oxygen species can induce changes in the antigenic structure of macromolecules, causing the immune system to treat them as “neo-antigens” and start production of autoantibodies. This study was designed to evaluate the relationship between oxidative stress markers, lifestyle factors and the detection of ANA.
Material and methods:
We examined measures of oxidative stress indices of free-radical damage to lipids and proteins, such as total oxidant status (TOS), concentration of protein thiol groups (PSH), and malondialdehyde (MDA), activity of superoxide dismutase (SOD) in in 1731 serum samples. The parameters of the non-enzymatic antioxidant system, such as total antioxidant status (TAS) and uric acid concentration (UA), were also measured and the oxidative stress index (OSI -index) was calculated. All samples were tested for the presence of ANA using an indirect immunofluorescence assay (IIFA).
Results:
The presence of ANA in women was associated with lower physical activity (p=0.036), less frequent smoking (p=0.007) and drinking of alcohol (p=0.024) accompanied by significant changes in SOD isoenzymes activity (p<0.001) and a higher uric acid (UA) concentration (p<0.001). In ANA positive males we observed lower concentrations of PSH (p=0.046) and increased concentrations of MDA (p=0.047).
Conclusions:
The results indicate that local oxidative stress may be associated with increased probability of ANA formation in a sex-specific manner.
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