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OBSTETRICS AND GYNAECOLOGY / BASIC RESEARCH
Analysis of lcnRNAHomebox transcript antisense RNA (HOTAIR) expression in Polish women with endometriosis
1
Department of Gynaecology, Oncological Gynaecology and Endometriosis Treatment, Polish Mother’s Memorial Hospital Research Institute, Lodz, Poland
2
Laboratory of Cancer Genetics, Department of Pathology, Polish Mother’s Memorial Hospital Research Institute, Lodz, Poland
3
Department of Science, Polish Mother’s Memorial Hospital Research Institute, Lodz, Poland
4
Department of Anaesthesiology and Operative Intensive Care Medicine, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin, Germany
Submission date: 2023-08-10
Final revision date: 2023-08-17
Acceptance date: 2023-08-17
Online publication date: 2023-08-22
Corresponding author
Beata Smolarz
Department of Pathology, Polish Mother’s Memorial Hospital Research Institute, 281/289 Rzgowska St, 93-338, Lodz, Poland
Department of Pathology, Polish Mother’s Memorial Hospital Research Institute, 281/289 Rzgowska St, 93-338, Lodz, Poland
Arch Med Sci 2025;21(2):688-694
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Long non-coding RNAs (lncRNAs) are implicated in endometriosis development. The aim of the study was to analyse the expression of lncRNA HOTAIR in the context of the risk of developing endometriosis.
Material and methods:
The presented study determined lncRNA HOTAIR expression in patients with endometriosis (n = 100) compared with a healthy control group (n = 100). Histological examination was performed by pathologists using a digital slide scanner and slide viewer software (Case Viewer 2.3, 3DHistech, Budapest, Hungary). The gene expression was measured by the reverse-transcription polymerase chain reaction (RT-PCR) technique. Two-step qRT-PCR was used to analyse the expression of the HOTAIR gene in normal tissue and in endometriosis samples. The total RNA was used for cDNA synthesis. Ct values were used for subsequent analysis, and GADPH was used as the endogenous control.
Results:
Statistically significantly higher HOTAIR expression was found in endometriosis samples compared to the expression in the control material (p < 0.0001). Regarding the classification of endometriosis (stage I–IV), there was a statistically significant increase in HOTAIR expression in stage IV (p < 0.05). There was no relationship between the age of the patients, the number of deliveries, the number of spontaneous abortions and BMI and the expression of HOTAIR.
Conclusions:
The reported studies suggest a significant role of lncRNA HOTAIR expression in the pathogenesis of endometriosis.
Long non-coding RNAs (lncRNAs) are implicated in endometriosis development. The aim of the study was to analyse the expression of lncRNA HOTAIR in the context of the risk of developing endometriosis.
Material and methods:
The presented study determined lncRNA HOTAIR expression in patients with endometriosis (n = 100) compared with a healthy control group (n = 100). Histological examination was performed by pathologists using a digital slide scanner and slide viewer software (Case Viewer 2.3, 3DHistech, Budapest, Hungary). The gene expression was measured by the reverse-transcription polymerase chain reaction (RT-PCR) technique. Two-step qRT-PCR was used to analyse the expression of the HOTAIR gene in normal tissue and in endometriosis samples. The total RNA was used for cDNA synthesis. Ct values were used for subsequent analysis, and GADPH was used as the endogenous control.
Results:
Statistically significantly higher HOTAIR expression was found in endometriosis samples compared to the expression in the control material (p < 0.0001). Regarding the classification of endometriosis (stage I–IV), there was a statistically significant increase in HOTAIR expression in stage IV (p < 0.05). There was no relationship between the age of the patients, the number of deliveries, the number of spontaneous abortions and BMI and the expression of HOTAIR.
Conclusions:
The reported studies suggest a significant role of lncRNA HOTAIR expression in the pathogenesis of endometriosis.
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| eISSN: | 1896-9151 |
| ISSN: | 1734-1922 |
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