ONCOLOGY / RESEARCH PAPER

Anti-human ovarian cancer, cytotoxicity, and antioxidant effects of Nigella Sativa green-formulated Au nanoparticles: Describing a new chemotherapeutic supplement
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Gynecology, The First People's Hospital of Wenling, Wenling, Zhejiang, 317500, China., China |
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Gynecology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, 250014, China., China |
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Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh -11451, Saudi Arabia., Saudi Arabia |
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Department of Pediatrics, College of Medicine and King Khalid University Hospital, King Saud University, Medical City, PO Box-2925, Riyadh -11461, Saudi Arabia., Saudi Arabia |
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Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, Texas, United States., United States |
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Gynecology, Northwest Women and Children's Hospital, Xi 'an, Shaanxi, 710061, China., China |
CORRESPONDING AUTHOR
Ling Zhang
Gynecology, Northwest Women and Children's Hospital, Xi 'an, Shaanxi, 710061, China., China
Submission date: 2021-05-30
Final revision date: 2021-07-22
Acceptance date: 2021-07-24
Online publication date: 2021-11-24
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ABSTRACT
Introduction:
The recent research showed that the Gold nanoparticles (GNPs) formulated with Nigella Sativa aqueous extract having potent antioxidant and anti-human ovarian cancer activities in vitro condition.
Material and methods:
For determinate the properties of the GNPs that were produced from the reaction between gold chloride solution with aqueous Nigella Sativa extract, we used UV–Visible Spectroscopy (UV-Vis), Field Emission Scanning Electron Microscopy (FE‐SEM), Fourier Transformed Infrared Spectroscopy (FT‐IR), and Transmission Electron Microscopy (TEM). For evaluating anti-ovarian cancer and cytotoxicity effects of GNPs, Au chloride, and Nigella Sativa aqueous extract, we used MTT assay.
Results:
The result of this test showed that GNPs have no cytotoxicity on normal cell line (HUVEC) and have potent anti-ovarian cancer features dose-dependently against PA-1, SK-OV-3, and SW-626 cell lines. The IC50 of GNPs were 249, 361, and 433 µg/mL against PA-1, SW-626, and SK-OV-3 cell lines, respectively. For evaluating the antioxidant features of GNPs, Au chloride, and Nigella Sativa aqueous extract, we used the DPPH test, in this test butylated hydroxytoluene was a positive control, the results of this test showed that the GNPs have an effective antioxidant feature. In the antioxidant test, the IC50 of GNPs and BHT were 144 and 201 µg/mL, respectively.
Conclusions:
Probably, potent anti-human ovarian cancer activities of GNPs formulated with Nigella Sativa aqueous seed extract because of antioxidant properties. After evaluating the effectiveness of this formulation in clinical trial researches, it can be a good alternative to chemotherapy drugs.