Current issue
Archive
Manuscripts accepted
About the Journal
Editorial office
Editorial board
Section Editors
Abstracting and indexing
Subscription
Contact
Ethical standards and procedures
Most read articles
Instructions for authors
Article Processing Charge (APC)
Regulations of paying article processing charge (APC)
NEPHROLOGY / BASIC RESEARCH
Long noncoding RNA MALAT1 can regulate proliferation and apoptosis of LPS-treated HK-2 cells via targeting miR-23a-3p through regulating ERK signaling
1
Department of Critical Care, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China
2
Department of Emergency, the First Hospital of JiLin University, Changchun, China
Submission date: 2020-02-18
Final revision date: 2020-03-24
Acceptance date: 2020-03-26
Online publication date: 2020-04-26
Publication date: 2025-04-23
Arch Med Sci 2025;21(2):630-637
KEYWORDS
TOPICS
ABSTRACT
Introduction:
The aim of the present study was to investigate the roles of long noncoding RNA (lncRNA) MALAT1 in the development of sepsis-induced acute kidney injury (septic AKI) and the underlying mechanism.
Material and methods:
The levels of MALAT1 in the serum of the septic AKI patients and healthy subjects were compared, and the targeting relationship between MALAT1 and miR-23a-3p was analyzed. Moreover, the effects of MALAT1 and miR-23a-3p on the proliferation and apoptosis of LPS-treated HK-2 cells were analyzed. Finally, the roles of ERK signaling during this process were analyzed.
Results:
We found that MALAT1 was markedly increased in serum of the septic AKI patients and LPS-treated cells. In addition, overexpression of MALAT1 relieved the injury induced by LPS in RMCs. Moreover, miR-23-a-3p has been confirmed as a target of MALAT1. Meanwhile, we also found that MALAT1 siRNA can increase the proliferation and inhibit the apoptosis of LPS-treated HK-2 cells through activating ERK signaling, and knockdown of miR-23a-3p can partially block the anti-apoptotic effect of MALAT1 siRNA.
Conclusions:
We report that MALAT1 can regulate the proliferation and apoptosis of LPS-treated HK-2 cells via targeting miR-23a-3p through regulating ERK signaling, suggesting that the MALAT1/miR-23a-3p axis could serve as a potential therapeutic target for the treatment of septic AKI.
The aim of the present study was to investigate the roles of long noncoding RNA (lncRNA) MALAT1 in the development of sepsis-induced acute kidney injury (septic AKI) and the underlying mechanism.
Material and methods:
The levels of MALAT1 in the serum of the septic AKI patients and healthy subjects were compared, and the targeting relationship between MALAT1 and miR-23a-3p was analyzed. Moreover, the effects of MALAT1 and miR-23a-3p on the proliferation and apoptosis of LPS-treated HK-2 cells were analyzed. Finally, the roles of ERK signaling during this process were analyzed.
Results:
We found that MALAT1 was markedly increased in serum of the septic AKI patients and LPS-treated cells. In addition, overexpression of MALAT1 relieved the injury induced by LPS in RMCs. Moreover, miR-23-a-3p has been confirmed as a target of MALAT1. Meanwhile, we also found that MALAT1 siRNA can increase the proliferation and inhibit the apoptosis of LPS-treated HK-2 cells through activating ERK signaling, and knockdown of miR-23a-3p can partially block the anti-apoptotic effect of MALAT1 siRNA.
Conclusions:
We report that MALAT1 can regulate the proliferation and apoptosis of LPS-treated HK-2 cells via targeting miR-23a-3p through regulating ERK signaling, suggesting that the MALAT1/miR-23a-3p axis could serve as a potential therapeutic target for the treatment of septic AKI.
REFERENCES (29)
1.
van Niekerk G, Meaker C, Engelbrecht AM: Nutritional support in sepsis: when less may be more. Crit Care 2020; 24: 53.
2.
Busch LM, Kalil AC, Kadri SS. Predicting sepsis mortality and costs using medicare claims: a method to the madness. Crit Care Med 2020; 48: 424-6.
3.
Seol CH, Yong SH, Shin JH, et al. The ratio of plasma angiopoietin-2 to angiopoietin-1 as a prognostic biomarker in patients with sepsis. Cytokine 2020; 129: 155029.
4.
Kazune S, Piebalga A, Strike E, Vanags I. Impaired vascular reactivity in sepsis – a systematic review with meta-analysis. Arch Med Sci Atheroscler Dis 2019; 4: e151-61.
5.
Girardot T, Schneider A, Rimmele T. Blood purification techniques for sepsis and septic AKI. Semin Nephrol 2019; 39: 505-14.
6.
Li YM, Zhang J, Su LJ, Kellum JA, Peng ZY. Downregulation of TIMP2 attenuates sepsis-induced AKI through the NF-kappaB pathway. Biochim Biophys Acta Mol Basis Dis 2019; 1865: 558-69.
7.
Genga KR, Trinder M, Kong HJ, et al. CETP genetic variant rs1800777 (allele A) is associated with abnormally low HDL-C levels and increased risk of AKI during sepsis. Sci Rep 2018; 8: 16764.
8.
Yang LG, Cao MZ, Zhang J, Li XY, Sun QL. LncRNA XIST modulates HIF-1A/AXL signaling pathway by inhibiting miR-93-5p in colorectal cancer. Mol Genet Genomic Med 2020; e1112.
9.
Chen L, Xiong Y, Yan C, et al. LncRNA KCNQ1OT1 accelerates fracture healing via modulating miR-701-3p/FGFR3 axis. FASEB J 2020; 34: 5208-22.
10.
Yang J, Lian Y, Yang R, et al. Upregulation of lncRNA LINC00460 facilitates GC progression through epigenetically silencing CCNG2 by EZH2/LSD1 and indicates poor outcomes. Mol Ther Nucleic Acids 2020; 19: 1164-75.
11.
Fan Y, Zhao X, Lu K, Cheng G. LncRNA BDNF-AS promotes autophagy and apoptosis in MPTP-induced Parkinson’s disease via ablating microRNA-125b-5p. Brain Res Bull 2020; 157: 119-27.
12.
Fang Q, Liu T, Yu C, et al. LncRNA TUG1 alleviates cardiac hypertrophy by targeting miR-34a/DKK1/Wnt-beta-catenin signalling. J Cell Mol Med 2020; 24: 3678-91.
13.
Jiang ZJ, Zhang MY, Fan ZW, Sun WL, Tang Y. Influence of lncRNA HOTAIR on acute kidney injury in sepsis rats through regulating miR-34a/Bcl-2 pathway. Eur Rev Med Pharmacol Sci 2019; 23: 3512-9.
14.
Shi S, Yang J, Fan W, Zhou Z, Chen G, Zhang J. Effects of LncRNA MALAT1 on microangiopathy and diabetic kidney disease in diabetic rats by regulating ERK/MAPK signaling pathway. Minerva Med 2019. doi: 10.23736/S0026-4806.19.06015-4.
15.
Ye Y, Zhang F, Chen Q, Huang Z, Li M. LncRNA MALAT1 modified progression of clear cell kidney carcinoma (KIRC) by regulation of miR-194-5p/ACVR2B signaling. Mol Carcinog 2019; 58: 279-92.
16.
Wu D, Cheng YG, Huang X, Zhong MW, Liu SZ, Hu SY. Downregulation of lncRNA MALAT1 contributes to renal functional improvement after duodenal-jejunal bypass in a diabetic rat model. J Physiol Biochem 2018; 74: 431-9.
17.
Liu P, Zhang B, Chen Z, et al. m(6)A-induced lncRNA MALAT1 aggravates renal fibrogenesis in obstructive nephropathy through the miR-145/FAK pathway. Aging (Albany NY) 2020; 12. doi: 10.18632/aging.102950.
18.
Fan H, Yuan J, Li X, et al. LncRNA LINC00173 enhances triple-negative breast cancer progression by suppressing miR-490-3p expression. Biomed Pharmacother 2020; 125: 109987.
19.
Yu Q, Zhao MW, Yang P. LncRNA UCA1 Suppresses the Inflammation via modulating miR-203-mediated regulation of MEF2C/NF-kappaB signaling pathway in epilepsy. Neurochem Res 2020. DOI: 10.1007/s11064-019-02952-9.
20.
Qin X, Zhu S, Chen Y, Chen D, Tu W, Zou H. Long non-coding RNA (LncRNA) CASC15 is upregulated in diabetes-induced chronic renal failure and regulates podocyte apoptosis. Med Sci Monit 2020; 26: e919415.
21.
da Cunha Jaeger M, Ghisleni EC, Cardoso PS, et al.: HDAC and MAPK/ERK inhibitors cooperate to reduce viability and stemness in medulloblastoma. J Mol Neurosci 2020. doi: 10.1007/s12031-020-01505-y.
22.
Poque E, Arnaud-Cormos D, Patrignoni L, et al. Effects of radiofrequency fields on RAS and ERK kinases activity in live cells using the bioluminescence resonance energy transfer technique. Int J Radiat Biol 2020. doi: 10.1080/09553002.2020.1730016.
23.
Du J, Lu Y, Song M, et al. Effects of ERK/p38 MAPKs signaling pathways on MTA-mediated osteo/odontogenic differentiation of stem cells from apical papilla: a vitro study. BMC Oral Health 2020; 20: 50.
24.
Zhou J, Fan Y, Zhong J, et al. TAK1 mediates excessive autophagy via p38 and ERK in cisplatin-induced acute kidney injury. J Cell Mol Med 2018; 22: 2908-921.
25.
Zhou W, Chen Y, Zhang X. Astragaloside IV alleviates lipopolysaccharide-induced acute kidney injury through down-regulating cytokines, CCR5 and p-ERK, and elevating anti-oxidative ability. Med Sci Monit 2017; 23: 1413-20.
26.
Wang S, Wei Q, Dong G, Dong Z. ERK-mediated suppression of cilia in cisplatin-induced tubular cell apoptosis and acute kidney injury. Biochim Biophys Acta 2013; 1832: 1582-90.
27.
Wani SJ, Mufti SA, Jan RA, et al. Combination of vitamin C, thiamine and hydrocortisone added to standard treatment in the management of sepsis: results from an open label randomised controlled clinical trial and a review of the literature. Infect Dis (Lond) 2020; 52: 271-8.
28.
Lindsell CJ, McGlothlin A, Nwosu S, et al. Update to the Vitamin C, Thiamine and Steroids in Sepsis (VICTAS) protocol: statistical analysis plan for a prospective, multicenter, double-blind, adaptive sample size, randomized, placebo-controlled, clinical trial. Trials 2019; 20: 670.
29.
Deutschman CS. Steroid responses in sepsis: some novel thinking that may provide new insight. Crit Care 2013; 17: 147.
Share
RELATED ARTICLE
| eISSN: | 1896-9151 |
| ISSN: | 1734-1922 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
