NEUROLOGY / RESEARCH PAPER
mRNA expression profiling of mitochondrial subunits in subjects with Parkinson’s disease
| 1 | Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 – 94018, Troina, Italy, Italy |
| 2 | Department of Neurology, Sant’Elia Hospital, Azienda Sanitaria Provinciale (ASP) Caltanissetta, Via Luigi Russo 6, 93100 Caltanissetta, Italy, Italy |
| 3 | Laboratory of Molecular Medicine and Genomics, Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi (SA), Italy, Italy |
| 4 | Genomix4Life Srl, Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, Baronissi (SA), Italy, Italy |
CORRESPONDING AUTHOR
Michele Salemi
Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 – 94018, Troina, Italy, Via Conte Ruggero, 94018, Troina, Italy
Oasi Research Institute-IRCCS, Troina. Via Conte Ruggero, 78 – 94018, Troina, Italy, Via Conte Ruggero, 94018, Troina, Italy
Submission date: 2020-09-08
Final revision date: 2020-11-27
Acceptance date: 2020-12-13
Online publication date: 2021-03-28
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Parkinson's disease (PD) is a common adult-onset neurodegenerative disorder caused by a progressive loss of dopaminergic neurons due to the accumulation of α-synuclein in the substantia nigra. Mitochondria are known to play a key role in cell respiratory function and bioenergetics. Indeed, mitochondrial dysfunction causes an insufficient energy production required to satisfy the needs of several organs, especially the nervous system. However, the profiling of messenger RNA (mRNA) expression of mitochondrial subunits in PD has not been systematically investigated yet.
Material and methods:
We explored the mRNA expression of mitochondrial DNA (mtDNA) encoded respiratory chain (RC) subunits in 43 PD patients and 43 normal controls (NC). Next generation sequencing analysis (NGS) and quantitative real-time Polymerase Chain Reaction (qRT-PCR) assay for the confirmation of the NGS results were used.
Results:
All tested mitochondrial RC subunits was significantly over-expressed in subjects with PD compared to NC. In qRT-PCR the mean expression of all mitochondrial subunits had an expression level of at least 7 times compared to NC.
Conclusions:
The over-expression of mitochondrial subunits in PD subjects with respect to NC might be secondary to a degeneration-related alteration of the mitochondrial structure and/or dynamics, or to the occurrence of a compensatory mechanism. The study of specific mRNA by peripheral blood mononuclear cells may provide a further diagnostic frame to early detect PD patients.
Parkinson's disease (PD) is a common adult-onset neurodegenerative disorder caused by a progressive loss of dopaminergic neurons due to the accumulation of α-synuclein in the substantia nigra. Mitochondria are known to play a key role in cell respiratory function and bioenergetics. Indeed, mitochondrial dysfunction causes an insufficient energy production required to satisfy the needs of several organs, especially the nervous system. However, the profiling of messenger RNA (mRNA) expression of mitochondrial subunits in PD has not been systematically investigated yet.
Material and methods:
We explored the mRNA expression of mitochondrial DNA (mtDNA) encoded respiratory chain (RC) subunits in 43 PD patients and 43 normal controls (NC). Next generation sequencing analysis (NGS) and quantitative real-time Polymerase Chain Reaction (qRT-PCR) assay for the confirmation of the NGS results were used.
Results:
All tested mitochondrial RC subunits was significantly over-expressed in subjects with PD compared to NC. In qRT-PCR the mean expression of all mitochondrial subunits had an expression level of at least 7 times compared to NC.
Conclusions:
The over-expression of mitochondrial subunits in PD subjects with respect to NC might be secondary to a degeneration-related alteration of the mitochondrial structure and/or dynamics, or to the occurrence of a compensatory mechanism. The study of specific mRNA by peripheral blood mononuclear cells may provide a further diagnostic frame to early detect PD patients.
