Basic research
BDNF and HSP gene polymorphisms and their influence on the progression of primary open-angle glaucoma in a Polish population
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Submission date: 2013-03-18
Final revision date: 2013-04-30
Acceptance date: 2013-05-30
Online publication date: 2014-09-05
Publication date: 2014-12-17
Arch Med Sci 2014;10(6):1206–1213
Introduction: Glaucoma is a neurodegenerative disease that is often associated with high intraocular pressure (IOP). One of the effects of elevated IOP is disorder of neurotrophic molecules transport, including BDNF and recruit specific cellular proteins called “heat shock proteins” (HSPs). The aim of this study was to evaluate a relationship between the BDNF and HSP70-1 gene polymorphisms with risk occurrence of primary open-angle glaucoma (POAG).
Material and methods: The study consisted of 167 patients with POAG (mean age 73 ±9) and 193 healthy subjects (mean age 64 ±13). Genomic DNA was extracted from peripheral blood. Analysis of the gene polymorphisms was performed using PCR-RFLP, using the following restriction enzymes: NlaIII (rs6265) and BsrBI (rs1043618). The Heidelberg Retinal Tomography (HRT) clinical parameters were also analyzed. The odds ratios (ORs) and 95% confidence intervals (CIs) for each genotype and allele were calculated.
Results: Comparison of the distributions of genotypes and alleles of the 196G/A polymorphism of the BDNF gene as well as 190G/C polymorphism of the HSP70-1 gene and analysis of the odds ratio (OR) showed no statistically significant differences between POAG patients and controls (p > 0.05). However, there was a statistically significant association of the 196G/A of BDNF and 190G/C of HSP70-1 gene polymorphisms with progression of POAG depending on values of clinical parameters. 196G/A of BDNF correlated with the parameters GDx and RA (p = 0.03; p = 0.002, respectively), while 190G/C of HSP70-1 correlated with c/d and RA (p = 0.014, p = 0.024, respectively).
Conclusions: The BDNF 196G/A and HSP70-1 190G/C gene polymorphisms may be related to progression of POAG.