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OBSTETRICS AND GYNAECOLOGY / CLINICAL RESEARCH
CBX3 promotes ovarian cancer progression by regulating p53/p21-mediated glucose metabolism via inhibiting NCOR2
1
Department of Gynecology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China
2
Department of Anesthesiology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China
3
Department of Obstetrics and Gynecology, Ningxia Peoples Hospital, Yinchuan, Ningxia, China
Submission date: 2021-08-13
Final revision date: 2022-04-07
Acceptance date: 2022-04-22
Online publication date: 2022-08-03
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Chromobox protein homolog 3 (CBX3) has been reported to play an oncogenic role in various tumors. Nevertheless, the role of CBX3 in ovarian cancer remains vague.
Material and methods:
Chromobox protein homolog 3 expression was determined by qRT-PCR and western blotting in ovarian cancer tissues and cell lines. Cell proliferation, cycle and apoptosis were detected using CCK-8 assay and flow cytometry. Transwell and wound healing assays were used to determine cell invasion and migration. Furthermore, the modulatory effects of CBX3 on NCOR2 expression and p53/p21-mediated glycolysis were confirmed.
Results:
The expression of CBX3 was significant elevated in ovarian cancer tissues and cell lines. CBX3 knockdown inhibited cell proliferation, invasion and migration, while it promoted G1/S phase blockade and cell apoptosis. Mechanism analysis verified that CBX3 downregulation increased NCOR2 expression and blocked subsequent p53/p21-mediated glycolysis. NCOR2 silencing and p53/p21 inhibitor treatment reversed the inhibitory effects of CBX3 knockdown on ovarian cancer cellular function.
Conclusions:
CBX3 promoted ovarian cancer progression by promoting p53/p21-mediated glycolysis via inhibiting NCOR2.
Chromobox protein homolog 3 (CBX3) has been reported to play an oncogenic role in various tumors. Nevertheless, the role of CBX3 in ovarian cancer remains vague.
Material and methods:
Chromobox protein homolog 3 expression was determined by qRT-PCR and western blotting in ovarian cancer tissues and cell lines. Cell proliferation, cycle and apoptosis were detected using CCK-8 assay and flow cytometry. Transwell and wound healing assays were used to determine cell invasion and migration. Furthermore, the modulatory effects of CBX3 on NCOR2 expression and p53/p21-mediated glycolysis were confirmed.
Results:
The expression of CBX3 was significant elevated in ovarian cancer tissues and cell lines. CBX3 knockdown inhibited cell proliferation, invasion and migration, while it promoted G1/S phase blockade and cell apoptosis. Mechanism analysis verified that CBX3 downregulation increased NCOR2 expression and blocked subsequent p53/p21-mediated glycolysis. NCOR2 silencing and p53/p21 inhibitor treatment reversed the inhibitory effects of CBX3 knockdown on ovarian cancer cellular function.
Conclusions:
CBX3 promoted ovarian cancer progression by promoting p53/p21-mediated glycolysis via inhibiting NCOR2.
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| eISSN: | 1896-9151 |
| ISSN: | 1734-1922 |
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