PULMONOLOGY / BASIC RESEARCH
Prognostic and diagnostic significance of Cavin 2 in lung adenocarcinoma
 
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1
Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital of Wuhan University, Wuhan City, Hubei, China
2
Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan City, Hubei, China
Submission date: 2018-11-04
Final revision date: 2019-01-27
Acceptance date: 2019-02-12
Online publication date: 2019-05-24
Publication date: 2020-08-06
 
Arch Med Sci 2020;16(5):1189–1195
 
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ABSTRACT
Introduction:
Cavin 2 down-regulation is reported in several malignant tumors and is associated with tumor progression. However, the role of Cavin 2 in lung adenocarcinoma is unknown. This study aimed to investigate the prognostic and diagnostic significance of Cavin 2 in lung adenocarcinoma.

Material and methods:
Cavin 2 expression levels were examined in 150 cases of lung adenocarcinoma and matched adjacent normal lung tissues using RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR), Western blotting and immunohistochemistry (IHC) assays. Then, the relationship of Cavin 2 expression with clinicopathological characteristics and patients’ survival was further evaluated in lung adenocarcinoma.

Results:
QPCR and Western blotting analysis indicated that Cavin 2 expression levels were significantly lower in lung adenocarcinoma tissues compared with those in adjacent normal lung tissues (p < 0.0001). The IHC results showed that positive expression of Cavin 2 was mainly located in cytoplasm as brown, but was hard to detect in lung adenocarcinoma tissues. The low-expression rates of Cavin 2 in lung adenocarcinoma and adjacent normal lung tissues were 62.0% and 20.0%, respectively, and the difference was significant (p < 0.0001). Lower expression of Cavin 2 was significantly associated with tumor size, TNM stage and lymph node metastasis (p < 0.05).

Conclusions:
Cavin 2 has low expression in lung adenocarcinoma, which might be regarded as a potential prognostic and diagnostic biomarker.

eISSN:1896-9151
ISSN:1734-1922