RHEUMATOLOGY / CLINICAL RESEARCH
The association between inefficient repair of oxidative DNA lesions and common polymorphisms of the key base excision repair genes as well as their expression levels in patients with rheumatoid arthritis
More details
Hide details
1
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Poland
2
Doctoral Study in Molecular Genetics, Cytogenetics and Medical Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Poland
3
Department of Rheumatology, Medical University of Lodz, Poland
4
National Geriatrics, Rheumatology and Rehabilitation Institute, Poland
5
Biobank, Department of Immunology and Allergy, Medical University of Lodz, Poland
6
Department of Pharmaceutical Microbiology and Biochemistry, Medical University of Lodz, Poland
Submission date: 2022-09-19
Final revision date: 2023-02-24
Acceptance date: 2023-04-09
Online publication date: 2023-05-04
Corresponding author
Tomasz Poplawski
Department of Pharmaceutical Microbiology and Biochemistry
Medical University of Lodz
Lodz, Poland
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Rheumatoid arthritis (RA) is a common autoimmune heterogeneous joint disease of still unknown etiology. The pathology of RA leads to chronic inflammation of the joint tissues, which causes joint cartilage and bone destruction. One of the characteristic features of RA is oxidative stress, most likely induced and stimulated by inappropriate B- and T-cell activity. This results in accumulation of oxidative DNA lesions in peripheral blood mononuclear cells (PBMCs) isolated from RA patients, as we have shown previously. We have hypothesized that oxidative stress together with an impaired DNA damage response (DDR) to oxidative DNA lesions (limited to base excision repair pathway – BER) may be responsible for increased incidence in RA patients of some diseases with a background of genetic instability such as lymphoma and lung cancer.
Material and methods:
Therefore, we determined the levels of oxidative DNA lesions and the kinetics of repair of DNA damage induced by tert-butyl hydroperoxide (TBH) in PBMCs of 30 RA patients and 30 healthy individuals. The metrics from the DNA damage and repair study were correlated with the genotypes of common polymorphisms of the key BER genes as well as their expression levels. DNA damage and repair were evaluated by alkaline single cell gel electrophoresis (comet assay), the genotypes of the polymorphism were determined by TaqMan SNP Genotyping Assay, and PrimeTime qPCR Assay was used to analyze the expression profile of genes related to BER.
Results:
We observed an association between RA occurrence and impaired DNA repair in PBMCs. After stratifying the subjects by quartiles of DNA repair efficiency observed in the controls, we found an association between increased risk of RA and inefficient DNA repair (OR and 95% CI: 2.4 and 0.34–17, 32 and 4.6–222.6, 104 and 8.5–1279.2, for the 2nd to 4th quartiles, respectively, compared with the 1st quartile). We also identified interactions between inefficient DNA repair and polymorphism of the UNG gene (rs246079), and lower expression of key BER genes – MUTH, NEIL3 and UNG.
Conclusions:
Our results suggest that the genetic variations within BER genes as well as epigenetic factors may be linked with RA by the modulation of the cellular response to oxidative stress. These polymorphisms may be a useful additional marker in this disease along with the genetic and/or environmental indicators of oxidative stress. However, these conclusions need to be validated in larger studies.
REFERENCES (42)
1.
Dayer JM. Aspects of resorption and formation of connective tissue during chronic inflammation in rheumatoid arthritis. Eur J Rheumatol Inflamm 1982; 5: 457-68.
2.
Batko B, Stajszczyk M, Świerkot J, et al. Prevalence and clinical characteristics of rheumatoid arthritis in Poland: a nationwide study. Arch Med Sci 2019; 15: 134-40.
3.
Dzięcioł-Anikiej Z, Kuryliszyn-Moskal A, Hryniewicz A, Kaniewska K, Chilińska-Kopko E, Dzięcioł J. Gait disturbances in patients with rheumatoid arthritis. Arch Med Sci 2020; 20: 1163-70.
4.
Karami J, Aslani S, Jamshidi A, Garshasbi M, Mahmoudi M. Genetic implications in the pathogenesis of rheumatoid arthritis: an updated review. Gene 2019; 702: 8-16.
5.
Nemtsova MV, Zaletaev DV, Bure IV, et al. Epigenetic changes in the pathogenesis of rheumatoid arthritis. Front Genet 2019; 10: 570.
6.
Dervišević A, Resić H, Sokolović Š, et al. Leptin is associated with disease activity but not with anthropometric indices in rheumatoid arthritis patients. Arch Med Sci 2018; 14: 1080-6.
7.
Scott IC, Steer S, Lewis CM, Cope AP. Precipitating and perpetuating factors of rheumatoid arthritis immunopathology: linking the triad of genetic predisposition, environmental risk factors and autoimmunity to disease pathogenesis. Best Pract Res Clin Rheumatol 2011; 25: 447-68.
8.
Kastbom A, Roos Ljungberg K, Ziegelasch M, Wetterö J, Skogh T, Martinsson K. Changes in anti-citrullinated protein antibody isotype levels in relation to disease activity and response to treatment in early rheumatoid arthritis. Clin Exp Immunol 2018; 194: 391-9.
9.
Huizinga TWJ, Amos CI, van der Helm-van Mil AHM, et al. Refining the complex rheumatoid arthritis phenotype based on specificity of the HLA-DRB1 shared epitope for antibodies to citrullinated proteins. Arthritis Rheum 2005; 52: 3433-8.
10.
van der Helm-van Mil AHM, Huizinga TWJ, de Vries RRP, Toes REM. Emerging patterns of risk factor make-up enable subclassification of rheumatoid arthritis. Arthritis Rheum 2007; 56: 1728-35.
11.
Scherer HU, Häupl T, Burmester GR. The etiology of rheumatoid arthritis. J Autoimmun 2020; 110: 102400.
12.
Sellam J, Rivière E, Courties A, et al. Serum IL-33, a new marker predicting response to rituximab in rheumatoid arthritis. Arthritis Res Ther 2016; 18: 294.
13.
Murdaca G, Greco M, Tonacci A, et al. IL-33/IL-31 axis in immune-mediated and allergic diseases. Int J Mol Sci 2019; 20: 5856.
14.
Macedo RBV, Kakehasi AM, de Andrade MVM. IL33 in rheumatoid arthritis: potential contribution to pathogenesis. Rev Bras Reumatol 2016; 56: 451-7.
15.
Mateen S, Moin S, Khan AQ, Zafar A, Fatima N. Increased reactive oxygen species formation and oxidative stress in rheumatoid arthritis. PLoS One 2016; 11: e0152925.
16.
Shah D, Wanchu A, Bhatnagar A. Interaction between oxidative stress and chemokines: possible pathogenic role in systemic lupus erythematosus and rheumatoid arthritis. Immunobiology 2011; 216: 1010-7.
17.
Kamata H, Honda SI, Maeda S, Chang L, Hirata H, Karin M. Reactive oxygen species promote TNFalpha-induced death and sustained JNK activation by inhibiting MAP kinase phosphatases. Cell 2005; 120: 649-61.
18.
Gao X, Xu X, Belmadani S, et al. TNF-alpha contributes to endothelial dysfunction by upregulating arginase in ischemia/reperfusion injury. Arterioscler Thromb Vasc Biol 2007; 27: 1269-75.
19.
Murdaca G, Spanò F, Cagnati P, Puppo F. Free radicals and endothelial dysfunction: potential positive effects of TNF- inhibitors. Redox Rep 2013; 18: 95-9.
20.
Mateen S, Moin S, Shahzad S, Khan AQ. Level of inflammatory cytokines in rheumatoid arthritis patients: correlation with 25-hydroxy vitamin D and reactive oxygen species. PLoS One 2017; 12: e0178879.
21.
Galita G, Brzezińska O, Gulbas I, et al. Increased sensitivity of PBMCs isolated from patients with rheumatoid arthritis to DNA damaging agents is connected with inefficient DNA repair. J Clin Med 2020; 9: E988.
22.
Mercer LK, Regierer AC, Mariette X, et al. Spectrum of lymphomas across different drug treatment groups in rheumatoid arthritis: a European Registries Collaborative Project. Ann Rheum Dis 2017; 76: 2025-30.
23.
Klungland A, Lindahl T. Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1). EMBO J 1997; 16: 3341-8.
24.
Krupa R, Sobczuk A, Popławski T, Wozniak K, Blasiak J. DNA damage and repair in endometrial cancer in correlation with the HOGG1 and RAD51 genes polymorphism. Mol Biol Rep 2011; 38: 1163-70.
25.
Poplawski T, Arabski M, Kozirowska D, et al. DNA damage and repair in gastric cancer--a correlation with the HOGG1 and RAD51 genes polymorphisms. Mutat Res 2006; 601: 83-91.
26.
Wozniak K, Szaflik JP, Zaras M, et al. DNA damage/repair and polymorphism of the HOGG1 gene in lymphocytes of AMD patients. J Biomed Biotechnol 2009; 2009: 827562.
27.
Montero-Melendez T, Perretti M. Gapdh gene expression is modulated by inflammatory arthritis and is not suitable for QPCR normalization. Inflammation 2014; 37: 1059-69.
28.
Tanaka A, To J, O’Brien B, Donnelly S, Lund M. Selection of reliable reference genes for the normalisation of gene expression levels following time course LPS stimulation of murine bone marrow derived macrophages. BMC Immunol 2017; 18: 43.
29.
Feng Q, Torii Y, Uchida K, Nakamura Y, Hara Y, Osawa T. Black tea polyphenols, theaflavins, prevent cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P450 1A1 in cell cultures. J Agric Food Chem 2002; 50: 213-20.
30.
Altieri F, Grillo C, Maceroni M, Chichiarelli S. DNA damage and repair: from molecular mechanisms to health implications. Antioxid Redox Signal 2008; 10: 891-937.
31.
Yamamoto F, Kasai H, Bessho T, et al. Ubiquitous presence in mammalian cells of enzymatic activity specifically cleaving 8-hydroxyguanine-containing DNA. Jpn J Cancer Res 1992; 83: 351-7.
32.
García S, Conde C. The role of poly(ADP-Ribose) polymerase-1 in rheumatoid arthritis. Mediators Inflamm 2015; 2015: 837250.
33.
Chen SY, Chen HH, Huang YC, et al. Polymorphism and protein expression of MUTYH gene for risk of rheumatoid arthritis. BMC Musculoskelet Disord 2017; 18: 69.
34.
Olive PL, Johnston PJ. DNA damage from oxidants: influence of lesion complexity and chromatin organization. Oncol Res 1997; 9: 287-94.
35.
Bahjat M, Guikema JEJ. The complex interplay between DNA injury and repair in enzymatically induced mutagenesis and DNA damage in B lymphocytes. Int J Mol Sci 2017; 18: E1876.
36.
Lou H, Pickering MC. Extracellular DNA and autoimmune diseases. Cell Mol Immunol 2018; 15: 746-55.
37.
Galita G, Sarnik J, Brzezinska O, et al. Polymorphisms in DNA repair genes and association with rheumatoid arthritis in a pilot study on a Central European population. J Mol Sci 2023; 24: 3804.
38.
Dudzińska D, Boncler M, Watala C. The cardioprotective power of leaves. Arch Med Sci 2015; 11: 819-39.
39.
Kaushik AS, Strath LJ, Sorge RE. Dietary interventions for treatment of chronic pain: oxidative stress and inflammation. Pain Ther 2020; 9: 487-98.
40.
Castañeda-Delgado JE, Macias-Segura N, Ramos-Remus C. Non-coding RNAs in rheumatoid arthritis: implications for biomarker discovery. Noncoding RNA 2022; 8: 35.
41.
Czochor J, Sulkowski P, Glazer PM. miR-155 overexpression promotes genomic instability by reducing high-fidelity polymerase delta expression and activating error-prone DSB repair. Mol Cancer Res 2016; 14: 363-73.
42.
Su LC, Huang AF, Jia H, Liu Y, Xu WD. Role of microRNA-155 in rheumatoid arthritis. Int J Rheum Dis 2017; 20: 1631-7.